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  • 产品名称：Mouse Apoe (Apolipoprotein E) Sandwich ELISA (STJE0004282)

  • 产品型号：STJE0004282
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse Apoe (Apolipoprotein E) Sandwich ELISA (STJE0004282)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.94ng/mL
Detection Limit: 1.56~100ng/mL
Short Description: This Apoe Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Apoe
Gene ID: 11816
Uniprot ID: APOE_MOUSE
Specificity: This kit recognizes Mouse ApoE in samples. No significant cross-reactivity or interference between Mouse ApoE and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells.
Protein Name Apolipoprotein E
Apo-E
Database Links Reactome: R-MMU-3000480
Reactome: -MMU-381426
Reactome: -MMU-8957275
Reactome: -MMU-8963888
Reactome: -MMU-8963901
Reactome: -MMU-8964026
Reactome: -MMU-8964058
Reactome: -MMU-975634
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Extracellular Vesicle
Endosome
Multivesicular Body
In The Plasma
Apoe Is Associated With Chylomicrons
Chylomicrons Remnants
Vldl
Ldl And Hdl Lipoproteins
Lipid Poor Oligomeric Apoe Is Associated With The Extracellular Matrix In A Calcium- And Heparan-Sulfate Proteoglycans-Dependent Manner
Lipidation Induces The Release From The Extracellular Matrix
Colocalizes With Cd63 And Pmel At Exosomes And In Intraluminal Vesicles Within Multivesicular Endosomes
Alternative ELISA Names Apolipoprotein E ELISA kit
Apo-E ELISA kit
Apoe ELISA kit
Specificity This kit recognizes Mouse ApoE in samples. No significant cross-reactivity or interference between Mouse ApoE and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-107 101
EDTA plasma (n=8) 90-106 97
Cell culture media (n=8) 92-104 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 5.30 15.90 50.00 5.40 16.60 53.60
Standard deviation 0.40 0.70 2.20 0.30 1.00 2.20
CV (%) 7.55 4.40 4.40 5.56 6.02 4.10
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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