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  • 产品名称：Mouse ARG1 (ArginaseI) Sandwich ELISA (STJE0019916)

  • 产品型号：STJE0019916
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse ARG1 (ArginaseI) Sandwich ELISA (STJE0019916)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 20.34 pg/mL
Detection Limit: 78.13-5000 pg/mL
Short Description: This ARG1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Arg1
Gene ID: 11846
Uniprot ID: ARGI1_MOUSE
Specificity: This kit recognizes Mouse ARG1 in samples. No significant cross-reactivity or interference between Mouse ARG1 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Expressed in macrophages. Expressed in precursor and mature group 2 innate lymphoid cells (ILC2s). Expressed in lung tumor-associated myeloid cells. Expressed in lung tumor-infiltrating dendritic cells.
Function Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively.the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. Plays a role in the immune response of alternatively activated or M2 macrophages in processes such as wound healing and tissue regeneration, immune defense against multicellular pathogens and parasites, and immune suppression and allergic inflammation.the regulatory outcome seems to be organ specific. In tumor-infiltrating dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs) plays a role in suppression of T cell-mediated antitumor immunity.
Protein Name Arginase-1
Liver-Type Arginase
Type I Arginase
Database Links Reactome: R-MMU-6798695
Reactome: -MMU-70635
Cellular Localisation Cytoplasm
Cytoplasmic Granule
Alternative ELISA Names Arginase-1 ELISA kit
Liver-Type Arginase ELISA kit
Type I Arginase ELISA kit
Arg1 ELISA kit
Specificity This kit recognizes Mouse ARG1 in samples. No significant cross-reactivity or interference between Mouse ARG1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-97 91
EDTA plasma (n=8) 91-107 99
Cell culture media (n=8) 84-98 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 331.49 926.71 3243.72 340.78 882.40 2996.82
Standard deviation 19.06 46.80 148.56 18.81 47.12 151.34
CV (%) 5.75 5.05 4.58 5.52 5.34 5.05
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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