产品详情
简单介绍:
Mouse Beta-EP (Beta-Endorphin) Competitive ELISA (STJE0004324)
详情介绍:
Applications: | ELISA |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 9.38pg/mL |
Detection Limit: | 15.63~1000pg/mL |
Short Description: | This Beta-EP Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 2.5h |
Detection: | Colormetric |
Gene Symbol: | Pomc |
Gene ID: | 18976 |
Uniprot ID: | COLI_MOUSE |
Specificity: | This kit recognizes Mouse Beta-EP in samples. No significant cross-reactivity or interference between Mouse Beta-EP and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Post Translational Modifications | Specific enzymatic cleavages at paired basic residues yield the different active peptides. |
Function | Corticotropin: Stimulates the adrenal glands to release cortisol. Melanocyte-stimulating hormone alpha: Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes. Melanocyte-stimulating hormone beta: Increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin: Endogenous orexigenic opiate. Met-enkephalin: Endogenous opiate. |
Protein Name | Pro-Opiomelanocortin Pomc Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma Gamma-Msh - Corticotropin Adrenocorticotropic Hormone Acth - Melanocyte-Stimulating Hormone Alpha Alpha-Msh Melanotropin Alpha - Corticotropin-Like Intermediary Peptide Clip - Lipotropin Beta Beta-Lph - Lipotropin Gamma Gamma-Lph - Melanocyte-Stimulating Hormone Beta Beta-Msh Melanotropin Beta - Beta-Endorphin - Met-Enkephalin |
Database Links | Reactome: R-MMU-111885 Reactome: -MMU-193048 Reactome: -MMU-194002 Reactome: -MMU-202040 Reactome: -MMU-209952 Reactome: -MMU-211976 Reactome: -MMU-375276 Reactome: -MMU-418555 Reactome: -MMU-418594 |
Cellular Localisation | Secreted Melanocyte-Stimulating Hormone Alpha And Beta-Endorphin Are Stored In Separate Granules In Hypothalamic Pomc Neurons Suggesting That Secretion May Be Under The Control Of Different Regulatory Mechanisms |
Alternative ELISA Names | Pro-Opiomelanocortin ELISA kit Pomc ELISA kit Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma ELISA kit Gamma-Msh - Corticotropin ELISA kit Adrenocorticotropic Hormone ELISA kit Acth - Melanocyte-Stimulating Hormone Alpha ELISA kit Alpha-Msh ELISA kit Melanotropin Alpha - Corticotropin-Like Intermediary Peptide ELISA kit Clip - Lipotropin Beta ELISA kit Beta-Lph - Lipotropin Gamma ELISA kit Gamma-Lph - Melanocyte-Stimulating Hormone Beta ELISA kit Beta-Msh ELISA kit Melanotropin Beta - Beta-Endorphin - Met-Enkephalin ELISA kit Pomc ELISA kit Pomc1 ELISA kit |
Specificity | This kit recognizes β-EP in samples. No significant cross-reactivity or interference between β-EP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item Specifications Storage
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type Range (%) Average Recovery (%)
Serum(n=8) | 89-105 | 96 |
EDTA plasma(n=8) | 85-101 | 92 |
Cell culture media(n=8) | 95-107 | 101 |
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 49.40 | 99.80 | 404.50 | 51.10 | 94.90 | 411.30 |
Standard deviation | 2.90 | 5.50 | 14.20 | 3.20 | 5.40 | 14.80 |
CV (%) | 5.87 | 5.51 | 3.51 | 6.26 | 5.69 | 3.60 |
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