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  • 产品名称：Human SAA1 (Serum amyloid A-1 protein) Sandwich ELISA (STJE0003512)

  • 产品型号：STJE0003512
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human SAA1 (Serum amyloid A-1 protein) Sandwich ELISA (STJE0003512)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.75ng/mL
Detection Limit: 1.25~80ng/mL
Short Description: This SAA1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: SAA1
Gene ID: 6288
Uniprot ID: SAA1_HUMAN
Specificity: This kit recognizes Human SAA in samples. No significant cross-reactivity or interference between Human SAA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
Function Major acute phase protein.
Protein Name Serum Amyloid A-1 Protein
Saa Cleaved Into - Amyloid Protein A
Amyloid Fibril Protein Aa - Serum Amyloid Protein A(2-104 - Serum Amyloid Protein A(3-104 - Serum Amyloid Protein A(2-103 - Serum Amyloid Protein A(2-102 - Serum Amyloid Protein A(4-101
Database Links Reactome: R-HSA-3000471
Reactome: R-HSA-416476
Reactome: R-HSA-418594
Reactome: R-HSA-444473
Reactome: R-HSA-445989
Reactome: R-HSA-6785807
Reactome: R-HSA-879415
Reactome: R-HSA-933542
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative ELISA Names Serum Amyloid A-1 Protein ELISA kit
Saa Cleaved Into - Amyloid Protein A ELISA kit
Amyloid Fibril Protein Aa - Serum Amyloid Protein A(2-104 - Serum Amyloid Protein A(3-104 - Serum Amyloid Protein A(2-103 - Serum Amyloid Protein A(2-102 - Serum Amyloid Protein A(4-101 ELISA kit
SAA1 ELISA kit
Specificity This kit recognizes Human SAA in samples. No significant cross-reactivity or interference between Human SAA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-95 90
EDTA plasma (n=8) 89-100 94
Cell culture media (n=8) 92-103 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 3.70 8.07 35.34 4.02 8.84 36.42
Standard deviation 0.23 0.46 1.55 0.21 0.43 1.65
CV (%) 6.22 5.70 4.39 5.22 4.86 4.53
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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