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  • 产品名称：Human SPTBN4 (Spectrin beta chain, non-erythrocytic 4) CLIA Kit (STJC0000978)

  • 产品型号：STJC0000978
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human SPTBN4 (Spectrin beta chain, non-erythrocytic 4) CLIA Kit (STJC0000978)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human SPTBN4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: SPTBN4
Gene ID: 57731
Uniprot ID: SPTN4_HUMAN
Specificity: This kit recognizes Human SPT Beta N4 in samples. No significant cross-reactivity or interference between Human SPT Beta N4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in skeletal muscle at the sarcolemma and in the muscle capillaries (at protein level). Abundantly expressed in brain and pancreatic islets.
Protein Name Spectrin Beta Chain - Non-Erythrocytic 4
Beta-Iv Spectrin
Spectrin - Non-Erythroid Beta Chain 3
Database Links Reactome: R-HSA-375165
Reactome: R-HSA-445095
Reactome: R-HSA-5673001
Reactome: R-HSA-6807878
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Alternative CLIA Names Spectrin Beta Chain - Non-Erythrocytic 4 CLIA kit
Beta-Iv Spectrin CLIA kit
Spectrin - Non-Erythroid Beta Chain 3 CLIA kit
SPTBN4 CLIA kit
KIAA1642 CLIA kit
SPTBN3 CLIA kit
Specificity This kit recognizes Human SPTβN4 in samples. No significant cross-reactivity or interference between Human SPTβN4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-112 105
EDTA plasma(n=8) 91-102 97
Cell culture media(n=8) 99-117 107

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 98.80 245.60 988.16 93.35 260.81 940.49
Standard deviation 11.41 19.26 81.33 10.62 27.88 95.18
CV (%) 11.55 7.84 8.23 11.38 10.69 10.12
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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