产品详情
简单介绍：
Human SUMO2 (Small ubiquitin-related modifier 2) CLIA Kit (STJC0000901)
详情介绍：
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 37.5pg/mL |
| Detection Limit: | 62.5~4000pg/mL |
| Short Description: | This human SUMO2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | SUMO2 |
| Gene ID: | 6613 |
| Uniprot ID: | SUMO2_HUMAN |
| Specificity: | This kit recognizes Human SUMO2 in samples. No significant cross-reactivity or interference between Human SUMO2 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Broadly expressed. |
| Post Translational Modifications | Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4. Cleavage of precursor form by SENP1 or SENP2 is necessary for function. Monoubiquitinated N-terminally by UBE2W, which primes it for RNF4-dependent polyubiquitination by the UBE2V1-UBE2N heterodimer. |
| Function | Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Plays a role in the regulation of sumoylation status of SETX. |
| Protein Name | Small Ubiquitin-Related Modifier 2 Sumo-2 Hsmt3 Smt3 Homolog 2 Sumo-3 Sentrin-2 Ubiquitin-Like Protein Smt3b Smt3b |
| Database Links | Reactome: R-HSA-196791 Reactome: R-HSA-3065676 Reactome: R-HSA-3065678 Reactome: R-HSA-3065679 Reactome: R-HSA-3108214 Reactome: R-HSA-3232118 Reactome: R-HSA-3899300 Reactome: R-HSA-4085377 Reactome: R-HSA-4090294 Reactome: R-HSA-4551638 Reactome: R-HSA-4570464 Reactome: R-HSA-4615885 Reactome: R-HSA-5693607 Reactome: R-HSA-5696395 |
| Cellular Localisation | Nucleus Pml Body |
| Alternative CLIA Names | Small Ubiquitin-Related Modifier 2 CLIA kit Sumo-2 CLIA kit Hsmt3 CLIA kit Smt3 Homolog 2 CLIA kit Sumo-3 CLIA kit Sentrin-2 CLIA kit Ubiquitin-Like Protein Smt3b CLIA kit Smt3b CLIA kit SUMO2 CLIA kit SMT3B CLIA kit SMT3H2 CLIA kit |
| Specificity | This kit recognizes Human SUMO2 in samples. No significant cross-reactivity or interference between Human SUMO2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 88-102 | 93 |
| EDTA plasma(n=8) | 92-108 | 99 |
| Cell culture media(n=8) | 86-98 | 92 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 192.57 | 464.23 | 1696.20 | 197.99 | 465.85 | 1849.81 |
| Standard deviation | 15.52 | 44.71 | 188.45 | 23.22 | 40.67 | 124.68 |
| CV (%) | 8.06 | 9.63 | 11.11 | 11.73 | 8.73 | 6.74 |
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