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  • 产品名称：Human TAF13 (Transcription initiation factor TFIID subunit 13) CLIA Kit (STJC0000468)

  • 产品型号：STJC0000468
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human TAF13 (Transcription initiation factor TFIID subunit 13) CLIA Kit (STJC0000468)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human TAF13 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: TAF13
Gene ID: 6884
Uniprot ID: TAF13_HUMAN
Specificity: This kit recognizes Human TAF13 in samples. No significant cross-reactivity or interference between Human TAF13 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF13, together with TAF11 and TBP, play key roles during promoter binding by the TFIID and TFIIA transcription factor complexes.
Protein Name Transcription Initiation Factor Tfiid Subunit 13
Transcription Initiation Factor Tfiid 18 Kda Subunit
Taf(Ii18
Tafii-18
Tafii18
Database Links Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-674695
Reactome: R-HSA-6804756
Reactome: R-HSA-6807505
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-75953
Reactome: R-HSA-76042
Cellular Localisation Nucleus
Alternative CLIA Names Transcription Initiation Factor Tfiid Subunit 13 CLIA kit
Transcription Initiation Factor Tfiid 18 Kda Subunit CLIA kit
Taf(Ii18 CLIA kit
Tafii-18 CLIA kit
Tafii18 CLIA kit
TAF13 CLIA kit
TAF2K CLIA kit
TAFII18 CLIA kit
Specificity This kit recognizes Human TAF13 in samples. No significant cross-reactivity or interference between Human TAF13 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-104 98
EDTA plasma(n=8) 87-99 94
Cell culture media(n=8) 86-99 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 215.92 504.85 1976.00 219.49 478.80 2115.10
Standard deviation 26.90 38.22 137.92 20.94 39.26 197.13
CV (%) 12.46 7.57 6.98 9.54 8.20 9.32
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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