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  • 产品名称：Mouse Bax (Bcl-2 Associated X Protein) CLIA Kit (STJC0001104)

  • 产品型号：STJC0001104
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse Bax (Bcl-2 Associated X Protein) CLIA Kit (STJC0001104)
详情介绍：
Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This mouse Bax kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Bax
Gene ID: 12028
Uniprot ID: BAX_MOUSE
Specificity: This kit recognizes Mouse Bax in samples. No significant cross-reactivity or interference between Mouse Bax and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in a wide variety of tissues.
Post Translational Modifications Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12.
Function Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production.
Protein Name Apoptosis Regulator Bax
Database Links Reactome: R-MMU-111457
Reactome: -MMU-114294
Reactome: -MMU-5620971
Reactome: -MMU-6804114
Cellular Localisation Isoform Alpha: Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Cytoplasm
Nucleus
Colocalizes With 14-3-3 Proteins In The Cytoplasm
Under Stress Conditions
Undergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion Membrane
Isoform Beta: Cytoplasm
Isoform Gamma: Cytoplasm
Alternative CLIA Names Apoptosis Regulator Bax CLIA kit
Bax CLIA kit
Specificity This kit recognizes Mouse Bax in samples. No significant cross-reactivity or interference between Mouse Bax and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-101 96
EDTA plasma(n=8) 98-114 104
Cell culture media(n=8) 97-113 104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 104.91 281.82 931.01 94.62 264.27 885.74
Standard deviation 11.09 22.71 77.74 7.59 23.92 89.64
CV (%) 10.57 8.06 8.35 8.02 9.05 10.12
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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