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  • 产品名称：Mouse ER Beta (Estrogen Receptor Beta) CLIA Kit (STJC0001216)

  • 产品型号：STJC0001216
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse ER Beta (Estrogen Receptor Beta) CLIA Kit (STJC0001216)
详情介绍：
Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This mouse ER Beta kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Esr2
Gene ID: 13983
Uniprot ID: ESR2_MOUSE
Specificity: This kit recognizes Mouse ER Beta in samples. No significant cross-reactivity or interference between Mouse ER Beta and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in prostate, ovary, Leydig cells and in epithelium of the efferent ductules and of the initial segment of the epididymis.
Post Translational Modifications Phosphorylation at Ser-87 and Ser-105 recruits NCOA1.
Function Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner.
Protein Name Estrogen Receptor Beta
Er-Beta
Nuclear Receptor Subfamily 3 Group A Member 2
Database Links Reactome: R-MMU-1257604
Reactome: -MMU-383280
Reactome: -MMU-6811558
Reactome: -MMU-8939211
Reactome: -MMU-9009391
Cellular Localisation Nucleus
Alternative CLIA Names Estrogen Receptor Beta CLIA kit
Er-Beta CLIA kit
Nuclear Receptor Subfamily 3 Group A Member 2 CLIA kit
Esr2 CLIA kit
Estrb CLIA kit
Nr3a2 CLIA kit
Specificity This kit recognizes Mouse ERβ in samples. No significant cross-reactivity or interference between Mouse ERβ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-107 101
EDTA plasma(n=8) 98-110 104
Cell culture media(n=8) 102-116 108

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 193.44 348.18 1679.62 200.28 324.69 1649.17
Standard deviation 18.67 35.83 187.11 24.41 32.60 179.92
CV (%) 9.65 10.29 11.14 12.19 10.04 10.91
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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