产品详情
简单介绍：
Mouse Fgf23 (Fibroblast growth factor 23) Sandwich ELISA (STJE0005180)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This Fgf23 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Fgf23 |
| Gene ID: | 64654 |
| Uniprot ID: | FGF23_MOUSE |
| Specificity: | This kit recognizes Mouse FGF23 in samples. No significant cross-reactivity or interference between Mouse FGF23 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases. O-glycosylated at Thr-171 and Thr-178 by GALNT3 and glycosylation of Thr-178 requires previous glycosylation at Thr171. Glycosylation is necessary for secretion.it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. Phosphorylation at Ser-180 mediated by FAM20C slows down glycosylation at Thr-178 notably. |
| Function | Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblasts differentiation and matrix mineralization. Up-regulates EGR1 expression in the presence of KL. |
| Protein Name | Fibroblast Growth Factor 23 Fgf-23 |
| Database Links | Reactome: R-MMU-109704 Reactome: -MMU-1257604 Reactome: -MMU-190322 Reactome: -MMU-190372 Reactome: -MMU-190373 Reactome: -MMU-190374 Reactome: -MMU-190375 Reactome: -MMU-381426 Reactome: -MMU-5654219 Reactome: -MMU-5654221 Reactome: -MMU-5654227 Reactome: -MMU-5654228 Reactome: -MMU-5654687 Reactome: -MMU-5654688 Reactome: -MMU-5654689 Reactome: -MMU-5654693 Reactome: -MMU-5654695 Reactome: -MMU-5654699 Reactome: -MMU-5654700 Reactome: -MMU-5654704 Reactome: -MMU-5654706 Reactome: -MMU-5654710 Reactome: -MMU-5654712 Reactome: -MMU-5654719 Reactome: -MMU-5654720 Reactome: -MMU-5654726 Reactome: -MMU-5654727 Reactome: -MMU-5654732 Reactome: -MMU-5654733 Reactome: -MMU-5658623 Reactome: -MMU-5673001 Reactome: -MMU-6811558 Reactome: -MMU-8957275 |
| Cellular Localisation | Secreted Secretion Is Dependent On O-Glycosylation |
| Alternative ELISA Names | Fibroblast Growth Factor 23 ELISA kit Fgf-23 ELISA kit Fgf23 ELISA kit |
| Specificity | This kit recognizes Mouse FGF23 in samples. No significant cross-reactivity or interference between Mouse FGF23 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 94-110 | 100 |
| EDTA plasma (n=8) | 95-110 | 102 |
| Cell culture media (n=8) | 94-108 | 102 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 45.50 | 88.07 | 434.38 | 49.92 | 90.33 | 426.98 |
| Standard deviation | 2.52 | 3.69 | 22.37 | 2.53 | 5.18 | 23.27 |
| CV (%) | 5.54 | 4.19 | 5.15 | 5.07 | 5.73 | 5.45 |
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