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  • 产品名称：Mouse HP (Haptoglobin) CLIA Kit (STJC0001269)

  • 产品型号：STJC0001269
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse HP (Haptoglobin) CLIA Kit (STJC0001269)
详情介绍：
Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38ng/mL
Detection Limit: 15.63~1000ng/mL
Short Description: This mouse HP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Hp
Gene ID: 15439
Uniprot ID: HPT_MOUSE
Specificity: This kit recognizes Mouse HP in samples. No significant cross-reactivity or interference between Mouse HP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed by the liver and secreted in plasma.
Function As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Protein Name Haptoglobin Cleaved Into - Haptoglobin Alpha Chain - Haptoglobin Beta Chain
Database Links Reactome: R-MMU-2168880
Reactome: -MMU-6798695
Cellular Localisation Secreted
Alternative CLIA Names Haptoglobin Cleaved Into - Haptoglobin Alpha Chain - Haptoglobin Beta Chain CLIA kit
Hp CLIA kit
Specificity This kit recognizes Mouse HP in samples. No significant cross-reactivity or interference between Mouse HP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 96
EDTA plasma(n=8) 90-102 95
Cell culture media(n=8) 98-115 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 47.56 156.33 472.54 47.06 168.86 461.74
Standard deviation 5.22 16.10 35.02 5.76 13.34 47.14
CV (%) 10.98 10.30 7.41 12.24 7.90 10.21
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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