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  • 产品名称：Mouse IL-12 (Interleukin 12) Sandwich ELISA (STJE0019718)

  • 产品型号：STJE0019718
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse IL-12 (Interleukin 12) Sandwich ELISA (STJE0019718)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19pg/mL
Detection Limit: 0.31-20pg/mL
Short Description: This IL-12 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Il12b
Il12a
Gene ID: 16160
16159
Uniprot ID: IL12B_MOUSE
IL12A_MOUSE
Specificity: This kit recognizes Mouse IL-12 in samples. No significant cross-reactivity or interference between Mouse IL-12 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Alternative ELISA Names Interleukin-12 Subunit Beta ELISA kit
Il-12b ELISA kit
Cytotoxic Lymphocyte Maturation Factor 40 Kda Subunit ELISA kit
Clmf P40 ELISA kit
Il-12 Subunit P40 ELISA kit
Il12b ELISA kit
Interleukin-12 Subunit Alpha ELISA kit
Il-12a ELISA kit
Cytotoxic Lymphocyte Maturation Factor 35 Kda Subunit ELISA kit
Clmf P35 ELISA kit
Il-12 Subunit P35 ELISA kit
Il12a ELISA kit
Specificity This kit recognizes Mouse IL-12 in samples. No significant cross-reactivity or interference between Mouse IL-12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-97 93
EDTA plasma (n=8) 86-98 90
Cell culture media (n=8) 93-105 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 0.94 2.22 8.84 0.83 2.17 8.86
Standard deviation 0.05 0.11 0.42 0.05 0.11 0.42
CV (%) 5.72 4.95 4.74 5.80 4.96 4.74
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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