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  • 产品名称：Mouse IL-4 (Interleukin 4) Sandwich ELISA (STJE0019960)

  • 产品型号：STJE0019960
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse IL-4 (Interleukin 4) Sandwich ELISA (STJE0019960)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.84 pg/mL
Detection Limit: 1.56-100 pg/mL
Short Description: This IL-4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Il4
Gene ID: 16189
Uniprot ID: IL4_MOUSE
Specificity: This kit recognizes Mouse IL-4 in samples. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Function Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning. Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells. Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6.
Protein Name Interleukin-4
Il-4
B-Cell Igg Differentiation Factor
B-Cell Growth Factor 1
B-Cell Stimulatory Factor 1
Bsf-1
Igg1 Induction Factor
Lymphocyte Stimulatory Factor 1
Database Links Reactome: R-MMU-6785807
Cellular Localisation Secreted
Alternative ELISA Names Interleukin-4 ELISA kit
Il-4 ELISA kit
B-Cell Igg Differentiation Factor ELISA kit
B-Cell Growth Factor 1 ELISA kit
B-Cell Stimulatory Factor 1 ELISA kit
Bsf-1 ELISA kit
Igg1 Induction Factor ELISA kit
Lymphocyte Stimulatory Factor 1 ELISA kit
Il4 ELISA kit
Il-4 ELISA kit
Specificity This kit recognizes Mouse IL-4 in samples. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-102 97
EDTA plasma (n=8) 88-100 95
Cell culture media (n=8) 93-105 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 4.96 10.06 42.93 4.50 10.75 38.74
Standard deviation 0.30 0.42 2.12 0.31 0.61 1.92
CV (%) 6.01 4.16 4.94 6.88 5.68 4.95
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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