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  • 产品名称：Mouse Il2ra (Interleukin-2 receptor subunit alpha) Sandwich ELISA (STJE0004983)

  • 产品型号：STJE0004983
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse Il2ra (Interleukin-2 receptor subunit alpha) Sandwich ELISA (STJE0004983)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This Il2ra Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Il2ra
Gene ID: 16184
Uniprot ID: IL2RA_MOUSE
Specificity: This kit recognizes Mouse IL-2R Alpha/CD25 in samples. No significant cross-reactivity or interference between Mouse IL-2R Alpha/CD25 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Interleukin-2 Receptor Subunit Alpha
Il-2 Receptor Subunit Alpha
Il-2-Ra
Il-2r Subunit Alpha
Il2-Ra
P55
Cd Antigen Cd25
Database Links Reactome: R-MMU-5673001
Reactome: -MMU-9020558
Reactome: -MMU-912526
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Interleukin-2 Receptor Subunit Alpha ELISA kit
Il-2 Receptor Subunit Alpha ELISA kit
Il-2-Ra ELISA kit
Il-2r Subunit Alpha ELISA kit
Il2-Ra ELISA kit
P55 ELISA kit
Cd Antigen Cd25 ELISA kit
Il2ra ELISA kit
Il2r ELISA kit
Specificity This kit recognizes Mouse Interleukin-2 Receptor alpha chain in samples. No significant cross-reactivity or interference between Mouse Interleukin-2 Receptor alpha chain and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-97 92
EDTA plasma (n=8) 94-109 99
Cell culture media (n=8) 86-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 246.98 579.84 2015.75 247.76 539.70 1990.30
Standard deviation 17.02 25.86 95.55 14.27 30.82 103.50
CV (%) 6.89 4.46 4.74 5.76 5.71 5.20
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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