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  • 产品名称：Mouse IRF3 (Interferon Regulatory Factor 3) Sandwich ELISA Kit (STJE0010058)

  • 产品型号：STJE0010058
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse IRF3 (Interferon Regulatory Factor 3) Sandwich ELISA Kit (STJE0010058)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.048ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This IRF3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Irf3
Gene ID: 54131
Uniprot ID: IRF3_MOUSE
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity
Post Translational Modifications Constitutively phosphorylated on many Ser/Thr residues. Activated following phosphorylation by TBK1 and IKBKE. Innate adapter protein MAVS, STING1 or TICAM1 are first activated by viral RNA, cytosolic DNA, and bacterial lipopolysaccharide (LPS), respectively, leading to activation of the kinases TBK1 and IKBKE. These kinases then phosphorylate the adapter proteins on the pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1. Phosphorylated IRF3 dissociates from the adapter proteins, dimerizes, and then enters the nucleus to induce IFNs. Ubiquitinated.ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated.ubiquitination involves TRIM21 leading to proteasomal degradation. Ubiquitinated by UBE3C, leading to its degradation. ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation. Proteolytically cleaved by apoptotic caspases during apoptosis, leading to its inactivation. Cleavage by CASP3 during virus-induced apoptosis inactivates it, preventing cytokine overproduction.
Function Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Protein Name Interferon Regulatory Factor 3
Irf-3
Database Links Reactome: R-MMU-1169408
Reactome: -MMU-1606341
Reactome: -MMU-168928
Reactome: -MMU-3134973
Reactome: -MMU-3134975
Reactome: -MMU-3270619
Reactome: -MMU-9013973
Reactome: -MMU-918233
Reactome: -MMU-933541
Reactome: -MMU-936440
Reactome: -MMU-936964
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Shuttles Between Cytoplasmic And Nuclear Compartments
With Export Being The Prevailing Effect
When Activated
Irf3 Interaction With Crebbp Prevents Its Export To The Cytoplasm
Recruited To Mitochondria Via Tomm70:Hsp90aa1 Upon Sendai Virus Infection
Alternative ELISA Names Interferon Regulatory Factor 3 ELISA kit
Irf-3 ELISA kit
Irf3 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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