产品详情
简单介绍：
Mouse OXA (Orexin A) Competitive ELISA (STJE0004835)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 37.5pg/mL |
| Detection Limit: | 62.5~4000pg/mL |
| Short Description: | This OXA Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 2.5h |
| Detection: | Colormetric |
| Gene Symbol: | Hcrt |
| Gene ID: | 15171 |
| Uniprot ID: | OREX_MOUSE |
| Specificity: | This kit recognizes Mouse OXA in samples. No significant cross-reactivity or interference between Mouse OXA and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Specific enzymatic cleavages at paired basic residues yield the different active peptides. |
| Function | Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A: Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. Orexin-B: Binds to orexin receptor HCRTR2/OX2R only. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. |
| Protein Name | Hypocretin Neuropeptide Precursor Hypocretin Hcrt Orexin Precursor Prepro-Orexin Preprohypocretin Cleaved Into - Orexin-A Hypocretin-1 Hcrt1 - Orexin-B Hypocretin-2 Hcrt2 |
| Database Links | Reactome: R-MMU-389397 Reactome: -MMU-416476 |
| Cellular Localisation | Rough Endoplasmic Reticulum Cytoplasmic Vesicle Synapse Associated With Perikaryal Rough Endoplasmic Reticulum As Well As Cytoplasmic Large Granular Vesicles At Synapses |
| Alternative ELISA Names | Hypocretin Neuropeptide Precursor ELISA kit Hypocretin ELISA kit Hcrt ELISA kit Orexin Precursor ELISA kit Prepro-Orexin ELISA kit Preprohypocretin Cleaved Into - Orexin-A ELISA kit Hypocretin-1 ELISA kit Hcrt1 - Orexin-B ELISA kit Hypocretin-2 ELISA kit Hcrt2 ELISA kit Hcrt ELISA kit Ox ELISA kit Ppox ELISA kit |
| Specificity | This kit recognizes OXA in samples. No significant cross-reactivity or interference between OXA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 93-108 | 99 |
| EDTA plasma(n=8) | 88-99 | 93 |
| Cell culture media(n=8) | 93-104 | 98 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 197.40 | 441.80 | 1403.30 | 188.10 | 428.50 | 1373.90 |
| Standard deviation | 12.60 | 19.90 | 60.30 | 10.00 | 23.60 | 52.20 |
| CV (%) | 6.38 | 4.50 | 4.30 | 5.32 | 5.51 | 3.80 |
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