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  • 产品名称：Mouse TOP1 (Topoisomerase I) Sandwich ELISA Kit (STJE0012733)

  • 产品型号：STJE0012733
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse TOP1 (Topoisomerase I) Sandwich ELISA Kit (STJE0012733)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.055ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This TOP1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Top1
Gene ID: 21969
Uniprot ID: TOP1_MOUSE
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity
Post Translational Modifications Sumoylated. Lys-119 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. Phosphorylation at Ser-508 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.
Function Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter.
Protein Name Dna Topoisomerase 1
Dna Topoisomerase I
Database Links Reactome: R-MMU-4615885
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Diffuse Nuclear Localization With Some Enrichment In Nucleoli
On Cpt Treatment
Cleared From Nucleoli Into Nucleoplasm
Sumoylated Forms Found In Both Nucleoplasm And Nucleoli
Alternative ELISA Names Dna Topoisomerase 1 ELISA kit
Dna Topoisomerase I ELISA kit
Top1 ELISA kit
Top-1 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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