产品详情
简单介绍:
Mouse Vip (VIP peptides) Competitive ELISA (STJE0005099)
详情介绍:
Applications: | ELISA |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 7.5pg/mL |
Detection Limit: | 12.5~800pg/mL |
Short Description: | This Vip Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 2.5h |
Detection: | Colormetric |
Gene Symbol: | Vip |
Gene ID: | 22353 |
Uniprot ID: | VIP_MOUSE |
Specificity: | This kit recognizes Mouse VIP in samples. No significant cross-reactivity or interference between Mouse VIP and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Protein Name | Vip Peptides Cleaved Into - Intestinal Peptide Phi-42 - Intestinal Peptide Phi-27 Peptide Histidine Isoleucinamide 27 - Vasoactive Intestinal Peptide Vip Vasoactive Intestinal Polypeptide |
Database Links | Reactome: R-MMU-418555 Reactome: -MMU-420092 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Vip Peptides Cleaved Into - Intestinal Peptide Phi-42 - Intestinal Peptide Phi-27 ELISA kit Peptide Histidine Isoleucinamide 27 - Vasoactive Intestinal Peptide ELISA kit Vip ELISA kit Vasoactive Intestinal Polypeptide ELISA kit Vip ELISA kit |
Specificity | This kit recognizes VIP in samples. No significant cross-reactivity or interference between VIP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 86-98 | 92 |
EDTA plasma(n=8) | 89-102 | 95 |
Cell culture media(n=8) | 95-105 | 100 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 41.60 | 92.10 | 385.20 | 45.70 | 89.50 | 349.50 |
Standard deviation | 2.50 | 4.90 | 20.00 | 2.50 | 4.90 | 15.00 |
CV (%) | 6.01 | 5.32 | 5.19 | 5.47 | 5.47 | 4.29 |
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