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  • 产品名称：Anti-Cleaved Caspase 3 (Asp175) Rabbit mAb

  • 产品型号：EB-AT0551
  • 产品厂商：CASMASCI
  • 产品文档：
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简单介绍：
Anti-Cleaved Caspase 3 (Asp175) Rabbit mAb Size: 100 ul Concentration: 1 mg/ml
详情介绍：

Anti-Cleaved Caspase 3 (Asp175) Rabbit mAb


Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Overview
Description
anti- Cleaved Caspase 3 (Asp175) rabbit monoclonal antibody
Reactivity
Human
Tested applications
WB: 1/1000, IHC: 1/50-1/200, ICC/IF: 1/50-1/200
Properties
Immunogen
Synthetic peptide mapping to the N-terminus adjacent to human active Caspase-3 (p17 subunit)
Clonality
Monoclonal, clone number: 4N10
Isotype
IgG
Form
Liquid
Preservative: 0.02% Sodium Azide
and 50% glycerol.
Storage instruction
Store at -20°C, Avoid freeze / thaw cycle. Do not aliquot the antibody.
Database links
Swiss Prot：P42574
Applications
WB Image


Western blot - Cleaved Caspase 3 antibody (EB-AT0551) at 1/1000 dilution
 
Sample: in Jurkat cell lysate treated with Camptothecin. 
 
Observed band size: 17kDa
 


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