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  • 产品名称：现货R&D货号M1000B Mouse IL-10 Quantikine ELISA Kit

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简单介绍：
现货R&D货号M1000B Mouse IL-10 Quantikine ELISA Kit 小鼠白介素10 elisa试剂盒，现货促销 现货R&D货号M1000B Mouse IL-10 Quantikine ELISA Kit
详情介绍：
现货R&D货号M1000B Mouse IL-10 Quantikine ELISA Kit 
小鼠白介素10 elisa试剂盒，现货促销
 
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    5.22 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse IL-10
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
 
Product Summary
The Quantikine Mouse IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-10 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-10 accurately. Results obtained using natural mouse IL-10 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-10.
 
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision over two lots of kit components. Assays were performed by at least three technicians.
 
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 76.3 123 815 71.4 115 809
Standard Deviation 2.56 2.85 17 5.39 7.64 55.5
CV% 3.4 2.3 2.1 7.5 6.6 6.9

Cell Culture Supernates, Serum
  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 26.8 75.5 585 26.2 80 583
Standard Deviation 2.5 6 29 1.7 6.2 31
CV% 9.3 7.9 5 6.5 7.8 5.3
 
 
Recovery

The recovery of IL-10 spiked to levels throughout the range of the assay was evaluated.

 

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 99 95-103
EDTA Plasma (n=4) 91 86-99
Heparin Plasma (n=4) 89 82-98
Serum (n=4) 89 85-96
 
Linearity
 
To assess the linearity of the assay, samples containing and/or spiked with various concentrations of mouse IL-10 in each matrix were diluted with Calibrator Diluent  and then assayed.
 
Mouse IL-10 Quantikine ELISA Kit
 
 
Preparation and Storage
 
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
 
 
Background: IL-10
 
Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
 
 
  • Long Name
    Interleukin 10
  • Entrez Gene IDs
    3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral);
  • Alternate Names

    CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF;

  • 现货R&D货号M1000B Mouse IL-10 Quantikine ELISA Kit 


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