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现货R&D货号MTA00B Mouse TNF-alpha Quantikine ELISA Kit 小鼠肿瘤坏死因子 α elisa试剂盒 现货促销5000元 现货R&D货号MTA00B Mouse TNF-alpha Quantikine ELISA Kit
详情介绍:
现货R&D货号MTA00B    Mouse TNF-alpha Quantikine ELISA Kit
小鼠肿瘤坏死因子 α  elisa试剂盒  现货促销5000元
现货R&D货号MTA00B    Mouse TNF-alpha Quantikine ELISA Kit
小鼠肿瘤坏死因子 α  elisa试剂盒  现货促销5000元
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    7.21 pg/mL
  • Assay Range
    10.9 - 700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse TNF-alpha.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
 
Product Summary
The Quantikine Mouse TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TNF-alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse TNF-alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse TNF-alpha accurately. Results obtained using natural mouse TNF-alpha showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TNF-alpha.
 
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision.
 
Cell Culture Supernates
  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 54.6 116 405 52.6 138 373
Standard Deviation 1.5 4.51 12.5 4.61 8.49 29.7
CV% 2.7 3.9 3.1 8.8 6.2 8

Serum, EDTA Plasma, Heparin Plasma
  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 50.5 134 375 53 146 388
Standard Deviation 1.91 4.95 11.5 3.69 8.42 29.7
CV% 3.8 3.7 3.1 7 5.8 7.7
 
 
Recovery

The recovery of mouse TNF-alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

 

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 104 94-111
EDTA Plasma (n=4) 95 85-103
Heparin Plasma (n=4) 92 86-98
Serum (n=4) 94 88-106
 
Linearity
 
To assess the linearity of the assay, samples were spiked with various concentrations of mouse TNF-alpha in each matrix, diluted with the appropriate Calibrator Diluent and then assayed.
 
Mouse TNF-alpha Quantikine ELISA Kit
 
 
Preparation and Storage
 
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
 
 
Background: TNF-alpha
 

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

 
 
  • Long Name
    Tumor Necrosis Factor alpha
  • Entrez Gene IDs
    7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit);
  • Alternate Names
    APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha;
现货R&D货号MTA00B    Mouse TNF-alpha Quantikine ELISA Kit
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